abstract：:Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...

journal_title：Biochimie

authors： Ronca F,Raggi A

更新日期：2015-11-01 00:00:00

abstract：:In the recent years, we have provided evidence that Tumor Protein 53-Induced Nuclear Protein 1 (TP53INP1) is a key stress protein with antioxidant-associated tumor suppressive function. The TP53INP1 gene, which is highly conserved in mammals, is over-expressed during stress responses including inflammation. This gene ...

journal_title：Biochimie

authors： Saadi H,Seillier M,Carrier A

更新日期：2015-11-01 00:00:00

abstract：:A novel antimicrobial peptide (AMP) was identified from the centipede Scolopendra subspinipes mutilans by RNA sequencing, and the amino acid sequences predicted from the sequenced mRNAs were compared with those of known AMPs. We named this peptide scolopendin, according to its origin, and investigated the molecular me...

journal_title：Biochimie

authors： Lee W,Hwang JS,Lee DG

更新日期：2015-11-01 00:00:00

abstract：:During clinical trials, a number of fully characterized molecules are dropped along the way because they do not provide enough benefit for the patient. Some of them show limited side effects and might be of great use for other applications. AS1411 is a nucleolin-targeting aptamer that underwent phase II clinical trial...

journal_title：Biochimie

authors： Métifiot M,Amrane S,Mergny JL,Andreola ML

更新日期：2015-11-01 00:00:00

abstract：:Diabetic cardiomyopathy is related to oxidative stress and correlated with the presence of advanced glycation end products (AGEs). In a clinical setting, AGEs can be detected in patients presenting diabetic cardiomyopathy; however, the underlying mechanism has yet to be elucidated. In our previous study, AGEs increase...

journal_title：Biochimie

authors： Ko SY,Chang SS,Lin IH,Chen HI

更新日期：2015-11-01 00:00:00

abstract：:A key target in the treatment of HIV-1/AIDS has been the viral protease. Here we first studied in silico the evolution of protease resistance. Primary active site resistance mutations were found to weaken interactions between protease and both inhibitor and substrate P4-P4' residues. We next studied the effects of sec...

journal_title：Biochimie

authors： Laco GS

更新日期：2015-11-01 00:00:00

abstract：UNLABELLED:Pancreatic cancer (PC) is one of the most lethal human malignancies and a major health problem. Patients diagnosed with PC and treated with conventional approaches have an overall 5-year survival rate of less than 5%. Novel strategies are needed to treat this disease. Herein, we propose a combinatorial strat...

journal_title：Biochimie

authors： Breton CS,Aubry D,Ginet V,Puyal J,Heulot M,Widmann C,Duchosal MA,Nahimana A

更新日期：2015-09-01 00:00:00

abstract：:Laccases, blue copper-containing oxidases, ≿ an play an important role in a variety of natural processes. The majority of fungal laccases are encoded by multigene families that express closely related proteins with distinct functions. Currently, only the properties of major gene products of the fungal laccase families...

journal_title：Biochimie

authors： Vasina DV,Mustafaev ON,Moiseenko KV,Sadovskaya NS,Glazunova OA,Tyurin АА,Fedorova TV,Pavlov AR,Tyazhelova TV,Goldenkova-Pavlova IV,Koroleva OV

更新日期：2015-09-01 00:00:00

abstract：:Disintegrins are small non-enzymatic proteins common in the venoms of many viperid snakes. These proteins have received significant attention due to their ability to inhibit platelet aggregation and cell adhesion, making them model compounds in drug development and design investigations. The present study used a combi...

journal_title：Biochimie

authors： Saviola AJ,Modahl CM,Mackessy SP

更新日期：2015-09-01 00:00:00

abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...

journal_title：Biochimie

authors： Postic G,Ghouzam Y,Gelly JC

更新日期：2015-08-01 00:00:00

abstract：:Despite its biological importance, iron is a pro-oxidant element and its accumulation results in tissue injury. Iron overload diseases such as thalassemia and hereditary hemochromatosis are commonly associated with liver tissue injury. Glutamyl cysteine (GC) is a dipeptide with antioxidant properties owing to its cyst...

journal_title：Biochimie

authors： Salama SA,Al-Harbi MS,Abdel-Bakky MS,Omar HA

更新日期：2015-08-01 00:00:00

abstract：:Translational control is a common mechanism used to regulate gene expression and occur in bacteria to mammals. Typically in translational control, an RNA-binding protein binds to a unique sequence in the mRNA to regulate protein synthesis by the ribosomes. Alternatively, a protein may bind to or modify a translation f...

journal_title：Biochimie

authors： Chen E,Joseph S

更新日期：2015-07-01 00:00:00

abstract：:Pentatricopeptide repeat (PPR) proteins are a large family of modular RNA-binding proteins which mediate several aspects of gene expression primarily in organelles but also in the nucleus. These proteins facilitate processing, splicing, editing, stability and translation of RNAs. While major advances in PPR research h...

journal_title：Biochimie

authors： Manna S

更新日期：2015-06-01 00:00:00

abstract：:Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...

journal_title：Biochimie

authors： Berger CM,Gaume X,Bouvet P

更新日期：2015-06-01 00:00:00

abstract：:Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, design...

journal_title：Biochimie

authors： Kato H,Gomez EA,Fujita M,Ishimaru Y,Uezato H,Mimori T,Iwata H,Hashiguchi Y

更新日期：2015-05-01 00:00:00

abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...

journal_title：Biochimie

authors： Farra R,Dapas B,Baiz D,Tonon F,Chiaretti S,Del Sal G,Rustighi A,Elvassore N,Pozzato G,Grassi M,Grassi G

更新日期：2015-05-01 00:00:00

abstract：:The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed...

journal_title：Biochimie

authors： Sharma P,Chinaranagari S,Chaudhary J

更新日期：2015-05-01 00:00:00

abstract：:The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases. The activation is auto-inhibited by a putative heli...

journal_title：Biochimie

authors： He P,Tan DL,Liu HX,Lv FL,Wu W

更新日期：2015-04-01 00:00:00

abstract：:Numerous studies have reported the beneficial effects of antioxidants in human diseases. Among their biological effects, a majority of antioxidants scavenge reactive radicals in the body, thereby reducing oxidative stress that is associated with the pathogenesis of many diseases. Antioxidant dendrimers are a new class...

journal_title：Biochimie

authors： Lee CY,Nanah CN,Held RA,Clark AR,Huynh UG,Maraskine MC,Uzarski RL,McCracken J,Sharma A

更新日期：2015-04-01 00:00:00

abstract：:The Cystic Fibrosis Transmembrane Regulator (CFTR) is a membrane protein whose mutations cause cystic fibrosis, a lethal genetic disease. We performed a molecular dynamic (MD) study of the properties of the nucleotide binding domains (NBD) whose conformational changes, upon ATP binding, are the direct responsible of t...

journal_title：Biochimie

authors： Belmonte L,Moran O

更新日期：2015-04-01 00:00:00

abstract：:Estrogen plays an essential role in bone formation, and estrogen modulation dysfunction is tightly associated with postmenopausal osteoporosis (PMOP). The underlying mechanisms of estrogen-mediated osteogenic differentiation have not been well defined. In this study, murine bone marrow mesenchymal stem cells were indu...

journal_title：Biochimie

authors： Gao B,Huang Q,Jie Q,Wang L,Zhang HY,Liu J,Yang L,Luo ZJ

更新日期：2015-03-01 00:00:00

abstract：:Mitochondria are essential organelles and consequently proper expression and maintenance of the mitochondrial genome are indispensable for proper cell function. The mitochondrial Suv3 (SUPV3L1) helicase is known to have a central role in mitochondrial RNA metabolism and to be essential for maintenance of mitochondrial...

journal_title：Biochimie

authors： Venø ST,Witt MB,Kulikowicz T,Bohr VA,Stevnsner T

更新日期：2015-01-01 00:00:00

abstract：:In recent years the chaperone HSC-70 has become a target for drug design with a strong focus in anticancer therapies. In our study of possible inhibitors of HSC-70 enzymatic activity we screened compounds by NMR as well as X-ray crystallography. As part of our screening efforts we crystallized the human HSC-70 ATP bin...

journal_title：Biochimie

authors： Zhang Z,Cellitti J,Teriete P,Pellecchia M,Stec B

更新日期：2015-01-01 00:00:00

abstract：:Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-pers...

journal_title：Biochimie

authors： Mohsen AW,Vockley J

更新日期：2015-01-01 00:00:00

abstract：:Caveolin is the protein marker of caveola-mediated endocytosis. Previously, we demonstrated by immunoblotting and immunofluorescence that an anti-chick embryo caveolin-1 monoclonal antibody (mAb) recognizes a protein in amoeba extracts. Nevertheless, the caveolin-1 gene is absent in the Entamoeba histolytica genome da...

journal_title：Biochimie

authors： Piña-Vázquez C,Reyes-López M,Mendoza-Hernández G,Bermúdez-Cruz RM,de la Garza M

更新日期：2014-12-01 00:00:00

abstract：:Alpha 1, 2-fucosyltransferase (FUT 1/2) is a rate-limiting enzyme that catalyzes the synthesis of Lewis y, a cell membrane-associated carbohydrate antigen. In human ovarian cancer, the upregulated expression of FUT1 and Lewis y is associated with advanced pathological stages and involved in cell proliferation, migrati...

journal_title：Biochimie

authors： Gao N,Liu J,Liu D,Hao Y,Yan L,Ma Y,Zhuang H,Hu Z,Gao J,Yang Z,Shi H,Lin B

更新日期：2014-12-01 00:00:00

abstract：:Plasmalogens (Pls) represent a specific subclass of glycerophospholipids characterized by the presence of a vinyl-ether bond at the sn-1 position of glycerol. Pls are quantitatively important in membranes of neuronal tissues, including the brain and the retina, where they can represent until almost two-third of ethano...

journal_title：Biochimie

authors： Saab S,Mazzocco J,Creuzot-Garcher CP,Bron AM,Bretillon L,Acar N

更新日期：2014-12-01 00:00:00

abstract：:The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...

journal_title：Biochimie

authors： Li X,Geng Z,Chang J,Song X,Wang Z

更新日期：2014-12-01 00:00:00

abstract：:Five 4-arylcoumarins (1c-g) and twelve 3,4-dihydro-4-arylcoumarins (2a-l) were synthesized and tested for antioxidant activity, antitumor activity, toxicity and structure-activity relationships analysis. 4-Arylcoumarins and 3,4-dihydro-4-arylcoumarins that possess two hydroxyl groups in ortho position, such as 1d, 1f,...

journal_title：Biochimie

authors： Zhang K,Ding W,Sun J,Zhang B,Lu F,Lai R,Zou Y,Yedid G

更新日期：2014-12-01 00:00:00

abstract：:A synthetic phosphonate inhibitor designed for lipase inhibition but displaying a broader range of activity was covalently immobilized on a solid support to generate a function-directed tool targeting serine hydrolases. To achieve this goal, straightforward and reliable analytical techniques were developed, allowing t...

journal_title：Biochimie

authors： Delorme V,Raux B,Puppo R,Leclaire J,Cavalier JF,Marc S,Kamarajugadda PK,Buono G,Fotiadu F,Canaan S,Carrière F

更新日期：2014-12-01 00:00:00

abstract：:The NADPH-oxidase complex, which plays beneficial or detrimental role in the inflammatory and degenerative diseases, is a membrane multi-subunit complex tightly regulated in order to produce superoxide anions, precursor of oxygen reactive species (ROS), in cells. The flavocytochrome b(558) (Cytb(558)) is the catalytic...

journal_title：Biochimie

authors： Souabni H,Machillot P,Baciou L

更新日期：2014-12-01 00:00:00

abstract：:p53 signaling plays an important role in cell death. Zerumbone, a natural cyclic sesquiterpene, has shown cytotoxic activity against many cancers. This study was done to investigate the anticancer effects of zerumbone on non-small cell lung cancer (NSCLC) cells and explored the involvement of p53 signaling. Cell viabi...

journal_title：Biochimie

authors： Hu Z,Zeng Q,Zhang B,Liu H,Wang W

更新日期：2014-12-01 00:00:00

abstract：:This review article aims to gather all the knowledge on two important proteins associated with Hevea brasiliensis rubber particles: namely the rubber elongation factor (REF) and the small rubber particle protein (SRPP). It covers more then three decades of research on these two proteins and their homologues in plants,...

journal_title：Biochimie

authors： Berthelot K,Lecomte S,Estevez Y,Peruch F

更新日期：2014-11-01 00:00:00

abstract：:When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF...

journal_title：Biochimie

authors： Kashima I,Takahashi M,Hashimoto Y,Sakota E,Nakamura Y,Inada T

更新日期：2014-11-01 00:00:00

abstract：:Nanoluciferase (NanoLuc) is a newly developed small luciferase reporter with the brightest bioluminescence reported to date. In the present work, we developed NanoLuc as a novel quantitative protein fusion tag for efficient overexpression in Escherichia coli and ultrasensitive bioluminescent assays using human leukemi...

journal_title：Biochimie

authors： He SX,Song G,Shi JP,Guo YQ,Guo ZY

更新日期：2014-11-01 00:00:00

abstract：:Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five α-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a si...

journal_title：Biochimie

authors： Arenas-Salinas M,Townsend PD,Brito C,Marquez V,Marabolli V,Gonzalez-Nilo F,Matias C,Watt RK,López-Castro JD,Domínguez-Vera J,Pohl E,Yévenes A

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting ...

journal_title：Biochimie

authors： He PP,Ouyang XP,Tang YY,Liao L,Wang ZB,Lv YC,Tian GP,Zhao GJ,Huang L,Yao F,Xie W,Tang YL,Chen WJ,Zhang M,Li Y,Wu JF,Peng J,Liu XY,Zheng XL,Yin WD,Tang CK

更新日期：2014-11-01 00:00:00

abstract：:As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...

journal_title：Biochimie

authors： Koh CY,Wetzel AB,de van der Schueren WJ,Hol WG

更新日期：2014-11-01 00:00:00

abstract：:LipA from Pseudomonas protegens Pf-5 has been proven not to be secreted into the extracytoplasmic space, proposing that it is a membrane protein in virtue of its N-terminal transmembrane domain predicted by the TMHMM 2.0. However, LipA was confirmed to be an intracellular protein through determining the effects of lip...

journal_title：Biochimie

authors： Zha D,Zhang H,Zhang H,Xu L,Yan Y

更新日期：2014-10-01 00:00:00

abstract：:NDPK-A, NDPK-B and NDPK-D are three enzymes which belong to the NDPK group I isoforms and are not only involved in metabolism process but also in transcriptional regulation, DNA cleavage, histidine protein kinase activity and metastasis development. Those enzymes were reported to bind to membranes either in mitochondr...

journal_title：Biochimie

authors： Francois-Moutal L,Marcillat O,Granjon T

更新日期：2014-10-01 00:00:00